| Effect of solvent extracts from papaya leaf on goat methane production and biohydrogenation of C18 PUFA |
| Paper ID : 1070-WDG2017 |
| Authors |
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Mahdi Ebrahimi *1, Saeid Jafari2, Goh Yong Meng3, Mohamed Ali Rajon3, Mohammad Faseleh Jahromi4, Arash Javanmrd3 1karaj, golshahr, west golzar, 2Post Doc 3Lecturer 4Post Doc |
| Abstract |
| Different solvents (hexane, chloroform, ethyl acetate, butanol, and water) were used to identify the effect of papaya leaf (PL) fractions (PLFs) on ruminal biohydrogenation (BH) and ruminal methanogenesis in an in vitro study. PLFs at a concentration of 0 (control, CON) and 15 mg/250 mg dry matter (DM) were mixed with 30 mL of buffered rumen fluid and were incubated for 24 h. Methane (CH4) production (mL/250 mg DM) was the highest (P < 0.05) for CON (7.65) and lowest for the chloroform fraction (5.41) compared to those of other PLFs at 24 h of incubation. Acetate to propionate ratio was the lowest for PLFs compared to that of CON. Supplementation of the diet with PLFs significantly (P < 0.05) decreased the rate of BH of C18:1n-9 (oleic acid; OA), C18:2n-6 (linoleic acid; LA), and C18:3n-3 (α-linolenic acid; LNA) compared to that of CON after 24 h of incubation. Real time PCR indicated that total protozoa and total methanogen population in PLFs decreased (P < 0.05) compared to those of CON. Keyword: Biohydrogenation, fermentation, papaya leaf fractions, methanogenesis, rumen. |
| Keywords |
| Biohydrogenation, fermentation, papaya leaf fractions, methanogenesis, rumen. |
| Status: Abstract Accepted (Poster Presentation) |