| Molecular analysis of Iranian goat breeds based on HVRI, cytB and ND6 regions of mitochondrial DNA |
| Paper ID : 1063-WDG2017 |
| Authors |
|
hamid reza seyedabadi * خ-شهید بهشتی روبروی دهقان ویلای اول |
| Abstract |
| Goat as a domestic animal has played a key role in food resources of Iranian plateau people and this area is known as the origin of this spice. Among the genetic markers mtDNA sequencing is one of the most useful and common methods employed for inferring phylogenetic relationship among closely related species and population and conservation of species. The purpose of this study was to determine the sequence of HVR1, cytB and ND6 genes of three native goat breeds of Iran (Marghoz, Mahabadi and Najdi). For this study 90 blood samples from unrelated sheep were collected. The DNA was extracted and these genes were amplified with specific primers using PCR. These regions of mtDNA of Iranian native goats were sequenced, alingned and compared with other breeds from all over the world. Results showed that Marghoz goat located in a separate lineage, Mahbadi goat is nearest to Turkish goat breed and Najdi goat is close to Chinese and Pakistani goats. Finally the cytB gene in Najdi Goat was registered in NCBI under accession number KY662383 and this breed was introduced to world for the first time. |
| Keywords |
| Iranian goat breeds, Mitochondrial genome, Sequencing |
| Status: Abstract Accepted (Poster Presentation) |