Investigation on the prevalence of Fasciola species in goat stool samples of East Azerbaijan Province by PCR-RFLP method
Paper ID : 1047-WDG2017
Authors:
Habib Cheraghi *1, خسرو پارسائی مهر2, سید عباس رأفت2, غلامعلی مقدم2, آرش جوانمرد3
1تبریز کوی دانشگاه 10 متری سیزدهم پلاک 19
2Department of Animal Science, Faculty of Agriculture, Tabriz University, Tabriz, Iran
3تبریز دانشگاه تبریز دانشکده کشاورزی گروه علوم دامی
Abstract:
Fasciolosis is one of the most common liver disease in humans and livestock, it is both economically and publicly important, and geographically, it has a global dispersal. Fasciola hepatica and Fasciola gigantica are known to cause fasciolosis. Each of which has a special epidemiological face. Recognition of Fasciola species has a significant role in choosing the correct methods for controlling the disease in a region. The aim of this study was to determine the species of Fasciola in fecal specimens of goats in East Azerbaijan Province by PCR-RFLP method. For this purpose, a total of 450 fecal samples were collected from different regions of East Azerbaijan province and After microscopic examination and ensuring the presence of Fasciola eggs in feces, sedimentation method removed the Fasciola eggs and a molecular study was performed on them. The results of the molecular analysis showed that all eggs belonged to the type of Fasciola hepatica in this study, there was no evidence of the presence of Fasciola gigantica egg infection in fecal specimens collected from ruminants in different parts of East Azerbaijan province.
Keywords:
Fasciolosis, Fasciola eggs, Goat, PCR-RFLP, East Azerbaijan
Status : Abstract Accepted (Poster Presentation)